Biopython

http://biopython.org/wiki/Main_Page

→ Install Biopython

→ get GC content

→ get inverse Sequence

→ Read/write fasta files

Remove duplicate sequences, remove too short sequences

http://biopython.org/wiki/Sequence_Cleaner

Extracting a subset of sequences

https://www.biostars.org/p/1709/

https://gist.github.com/brentp/477969

memory efficient reading of large data files

http://biopython.org/wiki/Split_large_file

Online services

access from python: NCBIblast, UniProt, KEGG, ChEMBL, Wikipathway

https://pypi.python.org/pypi/bioservices [ Bioservices documentation ]